Evaluación de la extracción subcrítica de fracciones acuosas de algas pardas presentes en el Caribe Colombianos: un avance hacia la consolidación de una biorrefinería

dc.contributor.advisorPuyana Hegedus, Mónica
dc.contributor.advisorPiñeros Castro, Nubia Yineth
dc.creatorGonzález Mejía, Juan Sebastián
dc.date.accessioned2026-03-16T22:51:51Z
dc.date.created2025-06-13
dc.description.abstractLas algas pardas de arribazón del género Sargassum se han convertido en una problemática de orden ambiental, económico y social en las playas del mar Caribe y el Golfo de México. Durante las arribazones masivas, las grandes masas de algas que terminan depositadas en la costa normalmente son incineradas o enterradas para evitar malos olores y afectaciones en la zona litoral. Los estudios sobre el aprovechamiento de este recurso en Colombia son escasos. Las algas de este género son una materia prima promisoria para la obtención de productos de alto valor como carbohidratos sulfatados, polifenoles, fucoxantina y ácidos grasos poliinsaturados como el Ácido docosahexaenoico (DHA), que, por sus importantes actividades biológicas, pueden ser usados en las industrias farmacéutica, cosmética, nutracéutica, entre otras. En este trabajo se evaluó la obtención de distintos compuestos bioactivos a partir de la extracción de sargazo de arribazón con agua subcrítica, bajo el concepto de biorrefinería, resultados que aportan al aprovechamiento de esta biomasa. Se realizaron experimentos utilizando un Diseño Compuesto Central Rotacional (DCCR) de dos variables, temperatura (90-180°C) y tiempo de extracción (30-90 min), teniendo como variables de respuesta la extracción de proteínas, carbohidratos, polifenoles y actividad antioxidante. Se encontró que los mejores resultados para la obtención de carbohidratos fueron 1h a 180°C y para polifenoles, con alta actividad antioxidante, 58 min a 198°C. Adicionalmente, se encontró que, mediante la extracción con agua subcrítica, no se logra una extracción eficiente de la fucoxantina. La extracción de la materia algal sin lavar es mayor respecto al material algal lavado previamente con agua dulce.
dc.description.abstractenglishBrown seaweed of the Sargassum genus have become an environmental, economic and social problem on beaches of the Caribbean Sea and the Gulf of Mexico. Massive blooms of Sargassum wash ashore and large masses of seaweed are usually incinerated or buried to avoid unpleasant odors and negative impacts on the coastal zone. Studies on the exploitation of this resource in Colombia are scarce. Algae of this genus are a promising material for obtaining high-value products such as sulfated carbohydrates, polyphenols, fucoxanthin, and polyunsaturated fatty acids like DHA, which, due to their significant biological activities, can be used in the pharmaceutical, cosmetic, and nutraceutical industries, among others. In this work, the production of various bioactive compounds from the extraction of beach-cast Sargassum with subcritical water, was evaluated under the concept of a biorefinery. These results contribute to the utilization of this biomass. Experiments were conducted using a two-variable Rotational Central Composite Design (RCCD), temperature (90-180°C) and extraction time (30-90 min), with extraction of proteins, carbohydrates, polyphenols and antioxidant activity as response variables. The best results for obtaining carbohydrates were found to be 1 h at 180°C, and for polyphenols, with high antioxidant activity, 58 min at 198°C. Additionally, it was found that extraction with subcritical water did not achieve an efficient extraction of fucoxanthin. Extraction of unwashed algal material is higher than that of algal material previously washed with fresh water.
dc.format.extent27 páginas
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dc.identifier.urihttps://hdl.handle.net/20.500.12010/39349
dc.language.isoes
dc.relation.referencesAlghoul, Z. M., Ogden, P. B., & Dorsey, J. G. (2017). Characterization of the polarity of subcritical water. Journal of Chromatography A, 1486, 42–49. DOI: 10.1016/J.CHROMA.2016.12.072
dc.relation.referencesarik, I. (2016). Magnetically modified Sargassum horneri biomass as an adsorbent for organic dye removal. Journal of Cleaner Production, 137(July), 189–194. DOI: 10.1016/j.jclepro.2016.07.068
dc.relation.referencesAnsari, F. A., Shriwastav, A., Gupta, S. K., Rawat, I., & Bux, F. (2017). Exploration of Microalgae Biorefinery by Optimizing Sequential Extraction of Major Metabolites from Scenedesmus obliquus. Industrial and Engineering Chemistry Research, 56(12), 3407–3412. DOI: 10.1021/acs.iecr.6b04814
dc.relation.referencesBula-Meyer, G. 2004. Las macroalgas marinas en la agronomía y el uso potencial del Sargassum flotante en la producción de fertilizantes en el archipiélago de San Andrés y Providencia, Colombia. Revista Intropica, 1: 91-103.
dc.relation.referencesCamacho, O. 2003. El género Sargassum C. Agardh 1820 (Phaeophyta-Fucales) del Parque Nacional Natural Tayrona, Caribe colombiano. Trabajo de Grado. Programa de Biología Marina, Universidad Jorge Tadeo Lozano, Santa Marta, Colombia, 107 pp.
dc.relation.referencesCamacho, O. & Hernández-Carmona, G. 2012. Phenology and alginates of two Sargassum species from the Caribbean coast of Colombia. Ciencias Marinas, 38(2): 381–393.
dc.relation.referencesCastro-Puyana, M., Herrero, M., Mendiola, J. A., & Ibáñez, E. (2013). Subcritical water extraction of bioactive components from algae. 534–560 p. En: Domínguez H. (ed.) Functional Ingredients from Algae for Foods and Nutraceuticals. Woodhead Publishing Series in Food Science, Technology and Nutrition. DOI: 10.1533/9780857098689.3.534
dc.relation.referencesConde, E., Moure, A. & Domínguez, H. (2015). Supercritical CO2 extraction of fatty acids, phenolics and fucoxanthin from freeze-dried Sargassum muticum. Journal of Applied Phycology, 27(2), 957–964. DOI: 10.1007/s10811-014-0389-0
dc.relation.referencesCosta, J.C., Oliveira, J.V., Pereira, M.A., Alves, M.M. & A. A. Abreu. 2015. Biohythane production from marine macroalgae Sargassum sp. coupling dark fermentation and anaerobic digestion, Bioresource Technology. Bioresource Technology, 190: 251-256. DOI: 10.1016/j.biortech.2015.04.052
dc.relation.referencesDubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350–356. DOI: 10.1021/AC60111A017/ASSET/AC60111A017.FP.PNG_V03
dc.relation.referencesEsquivel F. A., & Vargas Aguilar, P. (2007). Uso de aceites esenciales extraídos por medio de fluidos supercríticos para la elaboración de alimentos funcionales. Tecnología en Marcha, 20(4), 41-50.
dc.relation.referencesFranks, J. S., Johnson D. R. & Ko, D. S. (2016). Pelagic Sargassum in the Tropical North Atlantic. Gulf and Caribbean Research, 27 (1): SC6-SC11.
dc.relation.referencesFreestone D. & Morrison K.K. (2012). Current Legal Developments. The Sargasso Sea. The International Journal of Marine and Coastal Law, 27: 647–655. DOI: 10.1163/15718085-12341240
dc.relation.referencesFresneda, P. (2025). El sargazo llega en grandes cantidades a estas playas de Colombia: de dónde viene y cómo puede afectar. Diario AS S.L. Colombia. 28 de abril de 2025.
dc.relation.referencesGavio, B., Rincón-Díaz, M. N, & Santos-Martínez A. (2015). Massive quantities of pelagic Sargassum on the shores of San Andrés Island, southwestern Caribbean. Acta biol. Colomb., 20(1):239-241. DOI: 10.15446/abc.v20n1.46109.
dc.relation.referencesGifuni, I., Pollio, A., Safi, C., Marzocchella, A., & Olivieri, G. (2019). Current Bottlenecks and Challenges of the Microalgal Biorefinery. Trends in Biotechnology, 37(3), 242–252. DOI: 10.1016/j.tibtech.2018.09.006
dc.relation.referencesGower, J., Hu, C., Borstad, G. & King, S. (2006). Ocean color satellites show extensive lines of floating Sargassum in the Gulf of Mexico. IEEE Transactions on Geoscience and Remote Sensing, 44(22): 3619-3625.
dc.relation.referencesGower, J., Young, E. & King, S. (2013). Satellite images suggest a new Sargassum source region in 2011. Remote Sensing Letters, 4:8 764-773.
dc.relation.referencesGupta, S., & Abu-Ghannam, N. (2011a). Bioactive potential and possible health effects of edible brown seaweeds. Trends in Food Science and Technology, 22(6), 315–326. DOI: 10.1016/j.tifs.2011.03.011
dc.relation.referencesGupta, S., & Abu-Ghannam, N. (2011b). Recent developments in the application of seaweeds or seaweed extracts as a means for enhancing the safety and quality attributes of foods. Innovative Food Science and Emerging Technologies, 12(4), 600–609. DOI: 10.1016/j.ifset.2011.07.004
dc.relation.referencesHarb, T. B., Pereira, M. S., Cavalcanti, M. I. L. G., Fujii, M. T., & Chow, F. (2021). Antioxidant activity and related chemical composition of extracts from Brazilian beach-cast marine algae: opportunities of turning a waste into a resource. Journal of Applied Phycology, 33(5), 3383–3395. DOI: 10.1007/S10811-021-02446-8/METRICS
dc.relation.referencesHerrero, M., Cifuentes, A., & Ibañez, E. (2006). Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae: A review. Food Chemistry, 98(1), 136–148. DOI: 10.1016/J.FOODCHEM.2005.05.058
dc.relation.referencesJames, G., Stephenson, K., Callaghan-Gillespie, M., Kamara, M. T., Park, H. G., Brenna, J. T., & Manary, M. J. (2023). Docosahexaenoic Acid Stability in Ready-to-Use Therapeutic Food. Foods, 12(2), 308. DOI: 10.3390/FOODS12020308/S1
dc.relation.referencesJames-Cruz, J.L & Barrios-Torrejano, D.J. (2020). Valoración del uso del agua en la isla de San Andrés: turistas, hoteles y viviendas turísticas. PASOS-Revista de Turismo y Patrimonio Cultural., 18(2): 293-308. DOI: 10.25145/j.pasos.2020.18.020
dc.relation.referencesJohnson, D. R., Ko, D. S., Franks, J. S., Moreno, P. & Sanchez-Rubio, G. (2013). The Sargassum Invasion of the Eastern Caribbean and Dynamics of the Equatorial North Atlantic. GCFI, 65: 102-103.
dc.relation.referencesKadam, S. U., Álvarez, C., Tiwari, B. K., & O’Donnell, C. P. (2015). Extraction of biomolecules from seaweeds. 243-269 p. En: Tiwari B. K. & D. J. Troy (eds.) Seaweed Sustainability: Food and Non-Food Applications. Elsevier Inc. DOI: 10.1016/B978-0-12-418697-2.00009-X
dc.relation.referencesKawakami, K., Aketa, S., Nakanami, M., Iizuka, S., & Hirayama, M. (2010). Major water-soluble polyphenols, proanthocyanidins, in leaves of persimmon (Diospyros kaki) and their α-amylase inhibitory activity. Bioscience, Biotechnology and Biochemistry, 74(7), 1380–1385. DOI: 10.1271/BBB.100056
dc.relation.referencesKim, D.-Y., Vijayan, D., Praveenkumar, R., Han, J.-I., Lee, K., Park, J.-Y., Chang, W.-S., Lee, J.-S., & Oh, Y.-K. (2016). Cell-wall disruption and lipid/astaxanthin extraction from microalgae: Chlorella and Haematococcus. Bioresource Technology, 199, 300–310. DOI: 10.1016/j.biortech.2015.08.107
dc.relation.referencesKomoe, K., Sankare, Y., Fofie N.B.Y., Bamba A. & Sahr, A.G.-S. (2016). Taxonomic study of two species of Sargassum: Sargassum fluitans (Børgesen) Børgesen and Sargassum natans (Linnaneus) Gaillon (brown algae) collected in Côte d’Ivoire coasts, West Africa. Nature and Science, 14(10): 50-56
dc.relation.referencesLangin, K. (2018). Seaweed masses assault Caribbean islands. Science, 360(6394), 1157–1158. DOI: 10.1126/science.360.6394.1157
dc.relation.referencesLawag, I. L., Nolden, E. S., Schaper, A. A. M., Lim, L. Y., & Locher, C. (2023). A Modified Folin-Ciocalteu Assay for the Determination of Total Phenolics Content in Honey. Applied Sciences, 13(4): 2135. DOI: 10.3390/app13042135
dc.relation.referencesLouime, C., Fortune, J. & Gervais, G. (2017). Sargassum Invasion of Coastal Environments: A Growing Concern. American Journal of Environmental Sciences, 13(1): 58-64.
dc.relation.referencesMadeira, T., Marçal, C., Cardoso, S. M., Gando-Ferreira, L. M., & Costa, R. (2022). Ultrafiltration of Fucus vesiculosus Extracts Under Different Operating Conditions. Waste and Biomass Valorization, 13(11), 4447–4458. DOI: 10.1007/S12649-022-01807-9/METRICS
dc.relation.referencesMaeda, H., Hosokawa, M., Sashima, T., Funayama, K., & Miyashita, K. (2005). Fucoxanthin from edible seaweed, Undaria pinnatifida, shows antiobesity effect through UCP1 expression in white adipose tissues. Biochemical and Biophysical Research Communications, 332(2), 392–397. DOI: 10.1016/j.bbrc.2005.05.002
dc.relation.referencesMarín, A., Casas-Valdez, M., Carrillo, S., Hernández, H., Monroy, A., Sanginés, L., & Pérez-Gil, F. (2009). The marine algae Sargassum spp. (Sargassaceae) as feed for sheep in tropical and subtropical regions. Int. J. Trop. Biol., 57(4): 1271-1281
dc.relation.referencesMcLawrence, J.L.C, Sealy, H. & Roberts, D. (2017). The Impacts and Challenges of the 2015 Sargassum Seaweed Invasion in the Caribbean. International Journal of Ecology and Environmental Sciences, 43(4): 309-317.
dc.relation.referencesMichalak, I., & Chojnacka, K. (2014). Algal extracts: Technology and advances. Engineering in life sciences, 14(6), 581–591. DOI: 10.1002/elsc.201300055.
dc.relation.referencesMilledge, J. & Harvey, P. (2016). Golden Tides: Problem or Golden Opportunity? The valorisation of Sargassum from beach inundations. Journal of Marine Science and Engineering, 4(60): 1-19.
dc.relation.referencesMilledge, J. J., Nielsen, B. V., & Bailey, D. (2016). High-value products from macroalgae: the potential uses of the invasive brown seaweed, Sargassum muticum. Reviews in Environmental Science and Biotechnology, 15(1), 67–88. DOI: 10.1007/s11157-015-9381-7
dc.relation.referencesMiyashita, K., Mikami, N., & Hosokawa, M. (2013). Chemical and nutritional characteristics of brown seaweed lipids: A review. Journal of Functional Foods, 5(4), 1507–1517. DOI: 10.1016/j.jff.2013.09.019
dc.relation.referencesMoreira, A. & Alfonso, G. (2013). Inusual arribazón de Sargassum fluitans (Børgesen) Børgesen en la costa centro-sur de Cuba. Revista de Investigaciones Marinas, 33(2): 17-20.
dc.relation.referencesPádraigín, A. H. & FitzGerald, R. J. (2011). Bioactive Proteins, Peptides, and Amino Acids From Macroalgae J. Phycol. 47, 218–232. DOI: 10.1111/j.1529-8817.2011.00969.x
dc.relation.referencesParaguay-Delgado, F., Carreño-Gallardo, C., Estrada-Guel, I., Zabala-Arceo, A., Martinez-Rodriguez, H. A., & Lardizábal-Gutierrez, D. (2020). Pelagic Sargassum spp. capture CO2 and produce calcite. Environmental Science and Pollution Research, 27(20), 25794–25800. DOI: 10.1007/S11356-020-08969-W
dc.relation.referencesPérez-Larrán, P., Torres, M. D., Flórez-Fernández, N., Balboa, E. M., Moure, A., & Domínguez, H. (2019). Green technologies for cascade extraction of Sargassum muticum bioactives. Journal of Applied Phycology, 31(4), 2481–2495. DOI: 10.1007/s10811-018-1725-6
dc.relation.referencesPoojary, M. M., Barba, F. J., Aliakbarian, B., Donsì, F., Pataro, G., Dias, D. A., & Juliano, P. (2016). Innovative alternative technologies to extract carotenoids from microalgae and seaweeds. Marine Drugs, 14(11), 1–34. DOI: 10.3390/md14110214
dc.relation.referencesProtocol for Biuret Protein Assay - Creative Proteomics. (s/f). Recuperado el 7 de febrero de 2024, https://www.creative-proteomics.com/resource/protocol-for-biuret-protein-assay.htm
dc.relation.referencesRodríguez-Delgado, M. A., Malovaná, S., Pérez, J. P., Borges, T., & García Montelongo, F. J. (2001). Separation of phenolic compounds by high-performance liquid chromatography with absorbance and fluorimetric detection. Journal of Chromatography A, 912(2), 249–257. DOI: 10.1016/S0021-9673(01)00598-2
dc.relation.referencesRodríguez-Martínez, R.E., Tussenbroek, B. & Jordan-Dahlgren, E. (2016). Afluencia masiva de sargazo pelágico a la costa del Caribe Mexicano (2014-2015). Caribe Mexicano. 352-365 p. En: García-Mendoza, E., Quijano-Scheggia, S. I., Olivos-Ortiz, A., Núñez-Vázquez, E. J. (eds.) Florecimientos algales nocivos en México. Editorial CICESE, México.
dc.relation.referencesRodríguez-Martínez, R. E., & Van Tussenbroek, B. I. (2020). El sargazo en los pastos marinos y arrecifes. Revista Ciencia, 71(4): 28–33.
dc.relation.referencesSayre, R. M., Agin, P. P., LeVee, G. J., & Marlowe, E. (1979). A Comparison of In Vivo and In Vitro Testing of Sunscreening Formulas. Photochemistry and Photobiology, 29(3), 559–566. DOI: 10.1111/J.1751-1097.1979.TB07090.X
dc.relation.referencesSchell, J. M., Goodwin, D. S. & Siuda, A. N. S. (2015). Recent Sargassum inundation events in the Caribbean: Shipboard observations reveal dominance of a previously rare form. Oceanography, 28(3): 8–10.
dc.relation.referencesSissini, M. N., Barbosa, M. B, Menezes, M. T., Boucas, M., Cabral, M., Gower, J., Liu, G., De Oliveira, E., Milstein D., Gusmao, F., Martinelli-Filho, J. E., Alves-Lima, C., Colepicolo, P., Ameka, G., De Graftjohnson, K., Gouvea, L., Torrano-Silva, B., Nauer, F., De Castro, J.M., Bonomi, J., Rorig, L., Riosmena-Rodriguez, R., Mello T. J, Costa, L., & Antunes, P. (2017). The floating Sargassum (Phaeophyceae) of the South Atlantic Ocean-likely scenarios. Phycologia, 56(3): 321–328. DOI: 10.2216/16-92.1
dc.relation.referencesSluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., & Templeton, D. (2008). Determination of Ash in Biomass: Laboratory Analytical Procedure (LAP); Issue Date: 7/17/2005. www.nrel.gov
dc.relation.referencesSmetacek, V. & Zingone, A. (2013). Green and golden seaweed tides on the rise. Nature, 504: 84-88. DOI: 10.1038/nature12860
dc.relation.referencesSoto-Sierra, L., Stoykova, P., & Nikolov, Z. L. (2018). Extraction and fractionation of microalgae-based protein products. Algal Research, 36: 175–192. DOI: 10.1016/j.algal.2018.10.023
dc.relation.referencesStiger-Pouvreau, V., Mattio, L., De Ramon N’yeurt, A., Uwai, S., Dominguez, H., Flórez-Fernández, N., Connan, S., Critchley, A. T. (2023). A concise review of the highly diverse genus Sargassum C. Agardh with wide industrial potential. Journal Of Applied Phycology, 35(4): 1453-1483. DOI: 10.1007/s10811-023-02959-4
dc.relation.referencesTrivedi, J., Aila, M., Bangwal, D. P., Kaul, S., & Garg, M. O. (2015). Algae based biorefinery - How to make sense? Renewable and Sustainable Energy Reviews, 47, 295–307. DOI: 10.1016/j.rser.2015.03.052
dc.relation.referencesUrrea-Victoria, V., Nardelli, A. E., Floh, E. I. S., & Chow, F. (2020). Sargassum stenophyllum (Fucales, Ochrophyta) responses to temperature short-term exposure: photosynthesis and chemical composition. Revista Brasileira de Botanica, 43(4), 733–745. DOI: 10.1007/S40415-020-00639-Y/METRICS
dc.relation.referencesUsov, A. I., Bilan, M. I., Ustyuzhanina, N. E., & Nifantiev, N. E. (2022). Fucoidans of Brown Algae: Comparison of Sulfated Polysaccharides from Fucus vesiculosus and Ascophyllum nodosum. Marine Drugs 2022, Vol. 20, Page 638, 20(10), 638. DOI: 10.3390/MD20100638
dc.relation.referencesVasconcelos, J. B., de Vasconcelos, E. R. T. P. P., Urrea-Victoria, V., Bezerra, P. S., Reis, T. N. V., Cocentino, A. L. M., Navarro, D. M. A. F., Chow, F., Areces, A. J., & Fujii, M. T. (2019). Antioxidant activity of three seaweeds from tropical reefs of Brazil: potential sources for bioprospecting. Journal of Applied Phycology, 31(2), 835–846. DOI: 10.1007/S10811-018-1556-5/METRICS
dc.relation.referencesWang M., Hu, C., Cannizzaro, J., English, D., Han, X., Naar, D., Lapointe, B., Brewton, R., & Hernandez, F. (2018). Remote sensing of Sargassum biomass, nutrients, and pigments. Geophys. Res. Lett. 45, 12359–12367. DOI: 10.1029/2018GL078858
dc.relation.referencesWang, M., Hu C., Barnes, B. B., Mitchum, G., Lapointe, B. & Montoya, J. P. (2019). The great Atlantic Sargassum belt. Science, 365(6448): 83-87. DOI: 10.1126/science.aaw7912
dc.relation.referencesZayed, A., Muffler, K., Hahn, T., Rupp, S., Finkelmeier, D., Burger-Kentischer, A., & Ulber, R. (2016). Physicochemical and Biological Characterization of Fucoidan from Fucus vesiculosus Purified by Dye Affinity Chromatography. Marine Drugs, 14(4), 79. DOI: 10.3390/MD14040079
dc.relation.referencesZhao, D., Yu, D., Kim, M., Gu, M. Y., Kim, S. M., Pan, C. H., Kim, G. H., & Chung, D. (2019). Effects of temperature, light, and pH on the stability of fucoxanthin in an oil-in-water emulsion. Food Chemistry, 291, 87–93. DOI: 10.1016/J.FOODCHEM.2019.04.002
dc.subjectSargassum
dc.subjectAlgas de arribazón
dc.subjectBiorrefinería
dc.subjectAgua subcrítica
dc.subjectCarbohidratos
dc.subjectPolifenoles
dc.subjectActividad antioxidante
dc.subjectCaribe colombiano
dc.subject.keywordSargassum
dc.subject.keywordBeach-cast algae
dc.subject.keywordBiorefinery
dc.subject.keywordSubcritical water
dc.subject.keywordCarbohydrates
dc.subject.keywordPolyphenols
dc.subject.keywordAntioxidant activity
dc.subject.keywordColombian Caribbean
dc.subject.lembSargazo - Aprovechamiento
dc.subject.lembBiomasa - Procesamiento
dc.subject.lembCompuestos bioactivos - Extracción
dc.titleEvaluación de la extracción subcrítica de fracciones acuosas de algas pardas presentes en el Caribe Colombianos: un avance hacia la consolidación de una biorrefinería
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1

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