A high-throughput, multi-index isothermal amplification platform for rapid detection of 19 types of common respiratory viruses including SARS-CoV-2
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2020-09-05Autor
Xing, Wanli
Liu, Yingying
Wang, Huili
Li, Shanglin
Lin, Yongping
Chen, Lei
Zhao, Yan
Chao, Shuang
Huang, Xiaolan
Ge, Shaolin
Deng, Tao
Zhao, Tian
Li, Baolian
Wang, Hanbo
Wang, Lei
Song, Yunpeng
Jin, Ronghua
He, Jianxing
Zhao, Xiuying
Liu, Peng
Li, Weimin
Cheng, Jing
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Resumo em língua estrangeira
Fast and accurate diagnosis and the immediate isolation of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are regarded as the most effective measures to restrain the coronavirus disease 2019 (COVID-19) pandemic. Here, we present a high-throughput, multi-index nucleic acid isothermal amplification analyzer (RTisochipTM-W) employing a centrifugal microfluidic chip to detect 19 common respiratory viruses, including SARS-CoV-2, from 16 samples in a single run within 90 min. The limits of detection of all the viruses analyzed by the RTisochipTM-W system were equal to or less than 50 copies per microliter, which is comparable to those of conventional reverse-transcription polymerase chain reaction. We also demonstrate that the RTisochipTM-W system possesses the advantages of good repeatability, strong robustness, and high specificity. Finally, we analyzed 201 cases of preclinical samples, 14 cases of COVID-19-positive samples, 25 cases of clinically diagnosed samples, and 614 cases of clinical samples from patients or suspected patients with respiratory tract infections using the RTisochipTM-W system. The test results matched the referenced results well and reflected the epidemic characteristics of the respiratory infectious diseases. The coincidence rate of the RTisochipTM-W with the referenced kits was 98.15% for the detection of SARS-CoV-2. Based on these extensive trials, we believe that the RTisochipTM-W system provides a powerful platform for fighting the COVID-19 pandemic.
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https://www.sciencedirect.com/science/article/pii/S2095809920302381?via%3DihubCollections
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