A high-throughput, multi-index isothermal amplification platform for rapid detection of 19 types of common respiratory viruses including SARS-CoV-2

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dc.creatorXing, Wanli
dc.creatorLiu, Yingying
dc.creatorWang, Huili
dc.creatorLi, Shanglin
dc.creatorLin, Yongping
dc.creatorChen, Lei
dc.creatorZhao, Yan
dc.creatorChao, Shuang
dc.creatorHuang, Xiaolan
dc.creatorGe, Shaolin
dc.creatorDeng, Tao
dc.creatorZhao, Tian
dc.creatorLi, Baolian
dc.creatorWang, Hanbo
dc.creatorWang, Lei
dc.creatorSong, Yunpeng
dc.creatorJin, Ronghua
dc.creatorHe, Jianxing
dc.creatorZhao, Xiuying
dc.creatorLiu, Peng
dc.creatorLi, Weimin
dc.creatorCheng, Jing
dc.date.accessioned2020-09-17T23:58:28Z
dc.date.available2020-09-17T23:58:28Z
dc.date.created2020-09-05
dc.description.abstractenglishFast and accurate diagnosis and the immediate isolation of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are regarded as the most effective measures to restrain the coronavirus disease 2019 (COVID-19) pandemic. Here, we present a high-throughput, multi-index nucleic acid isothermal amplification analyzer (RTisochipTM-W) employing a centrifugal microfluidic chip to detect 19 common respiratory viruses, including SARS-CoV-2, from 16 samples in a single run within 90 min. The limits of detection of all the viruses analyzed by the RTisochipTM-W system were equal to or less than 50 copies per microliter, which is comparable to those of conventional reverse-transcription polymerase chain reaction. We also demonstrate that the RTisochipTM-W system possesses the advantages of good repeatability, strong robustness, and high specificity. Finally, we analyzed 201 cases of preclinical samples, 14 cases of COVID-19-positive samples, 25 cases of clinically diagnosed samples, and 614 cases of clinical samples from patients or suspected patients with respiratory tract infections using the RTisochipTM-W system. The test results matched the referenced results well and reflected the epidemic characteristics of the respiratory infectious diseases. The coincidence rate of the RTisochipTM-W with the referenced kits was 98.15% for the detection of SARS-CoV-2. Based on these extensive trials, we believe that the RTisochipTM-W system provides a powerful platform for fighting the COVID-19 pandemic.spa
dc.format.extent19 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1016/j.eng.2020.07.015spa
dc.identifier.issn2095-8099spa
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S2095809920302381?via%3Dihubspa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/13403
dc.language.isoengspa
dc.publisherEngineeringspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.localAbierto (Texto Completo)spa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectCOVID-19spa
dc.subject.keywordCoronavirus disease 2019spa
dc.subject.keywordSevere acute respiratory syndrome coronavirus 2spa
dc.subject.keywordMicrofluidicsspa
dc.subject.keywordNucleic acid testingspa
dc.subject.keywordIsothermal amplificationspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleA high-throughput, multi-index isothermal amplification platform for rapid detection of 19 types of common respiratory viruses including SARS-CoV-2spa
dc.type.coarhttp://purl.org/coar/resource_type/c_dcae04bcspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

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