Immune cell profiling of COVID-19 patients in the recovery stage by single-cell sequencing

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dc.creatorWen, Wen
dc.creatorSu, Wenru
dc.creatorTang, Hao
dc.creatorLe, Wenqing
dc.creatorZhang, Xiaopeng
dc.creatorZheng, Yingfeng
dc.creatorLiu, Xiuxing
dc.creatorXie, Lihui
dc.creatorLi, Jianmin
dc.creatorYe, Jinguo
dc.creatorDong, Liwei
dc.creatorCui, Xiuliang
dc.creatorMiao, Yushan
dc.creatorWang, Depeng
dc.creatorDong, Jiantao
dc.creatorXiao, Chuanle
dc.creatorChen, Wei
dc.creatorWang, Hongyang
dc.date.accessioned2020-08-25T14:00:29Z
dc.date.available2020-08-25T14:00:29Z
dc.date.created2020-05-04
dc.description.abstractenglishCOVID-19, caused by SARS-CoV-2, has recently affected over 1,200,000 people and killed more than 60,000. The key immune cell subsets change and their states during the course of COVID-19 remain unclear. We sought to comprehensively characterize the transcriptional changes in peripheral blood mononuclear cells during the recovery stage of COVID-19 by single-cell RNA sequencing technique. It was found that T cells decreased remarkably, whereas monocytes increased in patients in the early recovery stage (ERS) of COVID-19. There was an increased ratio of classical CD14++ monocytes with high inflammatory gene expression as well as a greater abundance of CD14++IL1β+ monocytes in the ERS. CD4+ T cells and CD8+ T cells decreased significantly and expressed high levels of inflammatory genes in the ERS. Among the B cells, the plasma cells increased remarkably, whereas the naïve B cells decreased. Several novel B cell-receptor (BCR) changes were identified, such as IGHV3-23 and IGHV3-7, and isotypes (IGHV3-15, IGHV3-30, and IGKV3-11) previously used for virus vaccine development were confirmed. The strongest pairing frequencies, IGHV3-23-IGHJ4, indicated a monoclonal state associated with SARS-CoV-2 specificity, which had not been reported yet. Furthermore, integrated analysis predicted that IL-1β and M-CSF may be novel candidate target genes for inflammatory storm and that TNFSF13, IL-18, IL-2, and IL-4 may be beneficial for the recovery of COVID-19 patients. Our study provides the first evidence of an inflammatory immune signature in the ERS, suggesting COVID-19 patients are still vulnerable after hospital discharge. Identification of novel BCR signaling may lead to the development of vaccines and antibodies for the treatment of COVID-19.spa
dc.format.extent18 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41421-020-0168-9spa
dc.identifier.issn2056-5968spa
dc.identifier.otherhttps://www.nature.com/articles/s41421-020-0168-9spa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/12214
dc.language.isoengspa
dc.publisherCell Discoveryspa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.rights.localAcceso restringidospa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectsingle-cell sequencingspa
dc.subjectImmune cell profiling of COVID-19 patientsspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleImmune cell profiling of COVID-19 patients in the recovery stage by single-cell sequencingspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

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