Infection of bat and human intestinal organoids by SARS-CoV-2

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dc.creatorZhou, Jie
dc.creatorLi, Cun
dc.creatorLiu, Xiaojuan
dc.creatorZhao, Xiaoyu
dc.creatorWang, Dong
dc.creatorWei, Yuxuan
dc.creatorLee, Andrew
dc.creatorZhang, Anna Jinxia
dc.creatorChu, Hin
dc.creatorCai, Jian Piao
dc.creatorYip, Cyril Chik Yan
dc.creatorChan, Ivy Hau Yee
dc.creatorWong, Kenneth Kak Yuen
dc.creatorTsang, Owen Tak Yin
dc.creatorChan, Kwok Hung
dc.creatorChan, Jasper Fuk Woo
dc.creatorTo, Kelvin Kai Wang
dc.creatorChen, Honglin
dc.creatorYuen, Kwok Yung
dc.date.accessioned2020-07-17T20:23:08Z
dc.date.available2020-07-17T20:23:08Z
dc.date.created2020-05-13
dc.description.abstractenglishA novel coronavirus—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally1,2. As of April 16, 2020, the confirmed case count of coronavirus disease 2019 (COVID-19) had surpassed 2 million. Based on full-genome sequence analysis, SARS-CoV-2 shows high homology to SARS-related coronaviruses identified in horseshoe bats1,2. Here we show the establishment and characterization of expandable intestinal organoids derived from horseshoe bats of the Rhinolophus sinicus species that can recapitulate bat intestinal epithelium. These bat enteroids are fully susceptible to SARS-CoV-2 infection and sustain robust viral replication. Development of gastrointestinal symptoms in some patients with COVID-19 and detection of viral RNA in fecal specimens suggest that SARS-CoV-2 might cause enteric, in addition to respiratory, infection3,4. Here we demonstrate active replication of SARS-CoV-2 in human intestinal organoids and isolation of infectious virus from the stool specimen of a patient with diarrheal COVID-19. Collectively, we established the first expandable organoid culture system of bat intestinal epithelium and present evidence that SARS-CoV-2 can infect bat intestinal cells. The robust SARS-CoV-2 replication in human intestinal organoids suggests that the human intestinal tract might be a transmission route of SARS-CoV-2.spa
dc.format.extent13 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41591-020-0912-6spa
dc.identifier.issn1546-170Xspa
dc.identifier.otherhttps://www.nature.com/articles/s41591-020-0912-6spa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/10806
dc.publisherScience Directeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectInfección de murciélagosspa
dc.subject.keywordInfection of batspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleInfection of bat and human intestinal organoids by SARS-CoV-2spa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

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