CRISPR–Cas12-based detection of SARS-CoV-2

dc.creatorBroughton, James P.
dc.creatorDeng, Xianding
dc.creatorYu, Guixia
dc.creatorFasching, Clare L.
dc.creatorServellita, Venice
dc.creatorSingh, Jasmeet
dc.creatorMiao, Xin
dc.creatorStreithorst, Jessica A.
dc.creatorGranados, Andrea
dc.creatorSotomayor-Gonzalez, Alicia
dc.creatorZorn, Kelsey
dc.creatorGopez, Allan
dc.creatorHsu, Elaine
dc.creatorGu, Wei
dc.creatorMiller, Steve
dc.creatorPan, Chao Yang
dc.creatorGuevara, Hugo
dc.creatorWadford, Debra A.
dc.creatorChen, Janice S.
dc.creatorChiu, Charles Y.
dc.date.accessioned2020-07-17T22:47:37Z
dc.date.available2020-07-17T22:47:37Z
dc.date.created2020-04-16
dc.description.abstractenglishAn outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.spa
dc.format.extent8 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41587-020-0513-4spa
dc.identifier.issn1546-1696spa
dc.identifier.otherhttps://www.nature.com/articles/s41587-020-0513-4spa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/10817
dc.publisherScience Directeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectCOVID-19spa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleCRISPR–Cas12-based detection of SARS-CoV-2spa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

Archivos

Bloque original

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
s41587-020-0513-4.pdf
Tamaño:
1.48 MB
Formato:
Adobe Portable Document Format
Descripción:
Documento reservado

Bloque de licencias

Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
license.txt
Tamaño:
2.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción: