CRISPR–Cas12-based detection of SARS-CoV-2
| dc.creator | Broughton, James P. | |
| dc.creator | Deng, Xianding | |
| dc.creator | Yu, Guixia | |
| dc.creator | Fasching, Clare L. | |
| dc.creator | Servellita, Venice | |
| dc.creator | Singh, Jasmeet | |
| dc.creator | Miao, Xin | |
| dc.creator | Streithorst, Jessica A. | |
| dc.creator | Granados, Andrea | |
| dc.creator | Sotomayor-Gonzalez, Alicia | |
| dc.creator | Zorn, Kelsey | |
| dc.creator | Gopez, Allan | |
| dc.creator | Hsu, Elaine | |
| dc.creator | Gu, Wei | |
| dc.creator | Miller, Steve | |
| dc.creator | Pan, Chao Yang | |
| dc.creator | Guevara, Hugo | |
| dc.creator | Wadford, Debra A. | |
| dc.creator | Chen, Janice S. | |
| dc.creator | Chiu, Charles Y. | |
| dc.date.accessioned | 2020-07-17T22:47:37Z | |
| dc.date.available | 2020-07-17T22:47:37Z | |
| dc.date.created | 2020-04-16 | |
| dc.description.abstractenglish | An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR–Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT–PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement. | spa |
| dc.format.extent | 8 páginas | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.doi | https://doi.org/10.1038/s41587-020-0513-4 | spa |
| dc.identifier.issn | 1546-1696 | spa |
| dc.identifier.other | https://www.nature.com/articles/s41587-020-0513-4 | spa |
| dc.identifier.uri | https://hdl.handle.net/20.500.12010/10817 | |
| dc.publisher | Science Direct | eng |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.source | reponame:Expeditio Repositorio Institucional UJTL | spa |
| dc.source | instname:Universidad de Bogotá Jorge Tadeo Lozano | spa |
| dc.subject | COVID-19 | spa |
| dc.subject.lemb | Síndrome respiratorio agudo grave | spa |
| dc.subject.lemb | COVID-19 | spa |
| dc.subject.lemb | SARS-CoV-2 | spa |
| dc.subject.lemb | Coronavirus | spa |
| dc.title | CRISPR–Cas12-based detection of SARS-CoV-2 | spa |
| dc.type.hasversion | info:eu-repo/semantics/acceptedVersion | spa |
| dc.type.local | Artículo | spa |
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