Detection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagents

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dc.creatorRocha, Alejandro Best
dc.creatorStroberg, Edana
dc.creatorBarton, Lisa M.
dc.creatorDuval, Eric J.
dc.creatorMukhopadhyay, Sanjay
dc.creatorYarid, Nicole
dc.creatorCaza, Tiffany
dc.creatorWilson, Jon D.
dc.creatorKenan, Daniel J.
dc.creatorKuperman, Michael
dc.creatorSharma, Shree G.
dc.creatorLarsen, Christopher P.
dc.date.accessioned2020-07-17T16:13:41Z
dc.date.available2020-07-17T16:13:41Z
dc.date.created2020-07-09
dc.description.abstractenglishCoronavirus Disease-19 (COVID-19), caused by the coronavirus SARS-CoV-2, was initially recognized in Wuhan, China and subsequently spread to all continents. The disease primarily affects the lower respiratory system, but may involve other organs and systems. Histopathologic evaluation of tissue from affected patients is crucial for diagnostic purposes, but also for advancing our understanding of the disease. For that reason, we developed immunohistochemical (IHC) and in situ hybridization (ISH) assays for detection of the. virus. A total of eight autopsy lungs, one placenta, and ten kidney biopsies from COVID-19 patients were stained with a panel of commercially available antibodies for IHC and commercially available RNA probes for ISH. Similarly, autopsy lungs, placentas and renal biopsies from non-COVID-19 patients were stained with the same antibodies and probes. All eight lungs and the placenta from COVID-19 patients stained positive by IHC and ISH, while the kidney biopsies stained negative by both methodologies. As expected, all specimens from non-COVID-19 patients were IHC and ISH negative. These two assays represent a sensitive and specific method for detecting the virus in tissue samples. We provide the protocols and the list of commercially available antibodies and probes for these assays, so they can be readily implemented in pathology laboratories and medical examiner offices for diagnostic and research purposes.spa
dc.format.extent5 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41374-020-0464-xspa
dc.identifier.issn1530-0307 (online)spa
dc.identifier.otherhttps://www.nature.com/articles/s41374-020-0464-xspa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/10753
dc.publisherLaboratory Investigationeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectDetection of SARS-CoV-2spa
dc.subjectHistopathologicspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleDetection of SARS-CoV-2 in formalin-fixed paraffin-embedded tissue sections using commercially available reagentsspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

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