Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform

Vista sencilla de ítem
dc.creatorThi Nhu Thao, Tran
dc.creatorLabroussaa, Fabien
dc.creatorEbert, Nadine
dc.creatorV’kovski, Philip
dc.creatorStalder, Hanspeter
dc.creatorPortmann, Jasmine
dc.creatorKelly, Jenna
dc.creatorSteiner, Silvio
dc.creatorHolwerda, Melle
dc.creatorKratzel, Annika
dc.creatorGultom, Mitra
dc.creatorSchmied, Kimberly
dc.creatorLaloli, Laura
dc.creatorHüsser, Linda
dc.creatorWider, Manon
dc.creatorPfaender, Stephanie
dc.creatorHirt, Dagny
dc.creatorCippà, Valentina
dc.creatorCrespo-Pomar, Silvia
dc.creatorSchröder, Simon
dc.creatorMuth, Doreen
dc.creatorNiemeyer, Daniela
dc.creatorCorman, Victor M.
dc.creatorMüller, Marcel A.
dc.creatorDrosten, Christian
dc.creatorDijkman, Ronald
dc.creatorJores, Joerg
dc.creatorThiel, Volker
dc.date.accessioned2020-07-21T16:47:14Z
dc.date.available2020-07-21T16:47:14Z
dc.date.created2020-05-04
dc.description.abstractenglishReverse genetics has been an indispensable tool to gain insights into viral pathogenesis and vaccine development. The genomes of large RNA viruses, such as those from coronaviruses, are cumbersome to clone and manipulate in Escherichia coli owing to the size and occasional instability of the genome1,2,3. Therefore, an alternative rapid and robust reverse-genetics platform for RNA viruses would benefit the research community. Here we show the full functionality of a yeast-based synthetic genomics platform to genetically reconstruct diverse RNA viruses, including members of the Coronaviridae, Flaviviridae and Pneumoviridae families. Viral subgenomic fragments were generated using viral isolates, cloned viral DNA, clinical samples or synthetic DNA, and these fragments were then reassembled in one step in Saccharomyces cerevisiae using transformation-associated recombination cloning to maintain the genome as a yeast artificial chromosome. T7 RNA polymerase was then used to generate infectious RNA to rescue viable virus. Using this platform, we were able to engineer and generate chemically synthesized clones of the virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)4, which has caused the recent pandemic of coronavirus disease (COVID-19), in only a week after receipt of the synthetic DNA fragments. The technical advance that we describe here facilitates rapid responses to emerging viruses as it enables the real-time generation and functional characterization of evolving RNA virus variants during an outbreak.spa
dc.format.extent24 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41586-020-2294-9spa
dc.identifier.issn1476-4687spa
dc.identifier.otherhttps://www.nature.com/articles/s41586-020-2294-9spa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/10866
dc.publisherScience Directeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectSARS-CoV-2spa
dc.subject.keywordSynthetic genomics platformspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleRapid reconstruction of SARS-CoV-2 using a synthetic genomics platformspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

Archivos

Bloque original

Mostrando 1 - 1 de 1
Miniatura
Nombre:
s41586-020-2294-9.pdf
Tamaño:
5.57 MB
Formato:
Adobe Portable Document Format
Descripción:
Documento reservado
Descargar

Bloque de licencias

Mostrando 1 - 1 de 1
Miniatura
Nombre:
license.txt
Tamaño:
2.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Documentos científicos relacionados a la COVID-19

Carrera 4 # 22-61 Teléfono: (+57) 601 242 7030 - 018000111022 Fax: (+57) 601 561 2107 Bogotá D.C., Colombia

Fundación Universitaria de Bogotá Jorge Tadeo Lozano | Vigilada Mineducación

Institución de educación superior privada, de utilidad común, sin ánimo de lucro y su carácter académico es el de Universidad

Reconocimiento personería jurídica: Resolución 2613 del 14 de agosto de 1959 Minjusticia.

Institución de Educación Superior sujeta a inspección y vigilancia por el Ministerio de Educación Nacional.

Software DSpace copyright © 2002-2026 LYRASIS