Structure of replicating SARS-CoV-2 polymerase

Hillen, Hauke S.; Kokic, Goran; Farnung, Lucas; Dienemann, Christian; Tegunov, Dimitry; Cramer, Patrick

doi:https://doi.org/10.1038/s41586-020-2368-8

Structure of replicating SARS-CoV-2 polymerase

dc.creator	Hillen, Hauke S.
dc.creator	Kokic, Goran
dc.creator	Farnung, Lucas
dc.creator	Dienemann, Christian
dc.creator	Tegunov, Dimitry
dc.creator	Cramer, Patrick
dc.date.accessioned	2020-07-21T14:03:45Z
dc.date.available	2020-07-21T14:03:45Z
dc.date.created	2020-05-21
dc.description.abstractenglish	The coronavirus SARS-CoV-2 uses an RNA-dependent RNA polymerase (RdRp) for the replication of its genome and the transcription of its genes1–3. Here we present the cryo-electron microscopic structure of the SARS-CoV-2 RdRp in active form, mimicking the replicating enzyme. The structure comprises the viral proteins nsp12, nsp8, and nsp7, and over two turns of RNA template-product duplex. The active site cleft of nsp12 binds the first turn of RNA and mediates RdRp activity with conserved residues. Two copies of nsp8 bind to opposite sides of the cleft and position the second turn of RNA. Long helical extensions in nsp8 protrude along exiting RNA, forming positively charged ‘sliding poles’. These sliding poles can account for the known processivity of the RdRp that is required for replicating the long coronavirus genome3. Our results enable a detailed analysis of the inhibitory mechanisms that underlie the antiviral activity of substances such as remdesivir, a drug for the treatment of coronavirus disease 2019 (COVID-19)4.	spa
dc.format.extent	15 páginas	spa
dc.format.mimetype	application/pdf	spa
dc.identifier.doi	https://doi.org/10.1038/s41586-020-2368-8	spa
dc.identifier.issn	1476-4687	spa
dc.identifier.other	https://www.nature.com/articles/s41586-020-2368-8	spa
dc.identifier.uri	https://hdl.handle.net/20.500.12010/10840
dc.publisher	Science Direct	eng
dc.rights.accessrights	info:eu-repo/semantics/openAccess	spa
dc.source	reponame:Expeditio Repositorio Institucional UJTL	spa
dc.source	instname:Universidad de Bogotá Jorge Tadeo Lozano	spa
dc.subject	Polimerasa	spa
dc.subject.keyword	Polymerase	spa
dc.subject.lemb	Síndrome respiratorio agudo grave	spa
dc.subject.lemb	COVID-19	spa
dc.subject.lemb	SARS-CoV-2	spa
dc.subject.lemb	Coronavirus	spa
dc.title	Structure of replicating SARS-CoV-2 polymerase	spa
dc.type.hasversion	info:eu-repo/semantics/acceptedVersion	spa
dc.type.local	Artículo	spa

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