Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

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dc.creatorVogels, Chantal B. F.
dc.creatorBrito, Anderson F.
dc.creatorWyllie, Anne L.
dc.creatorFauver, Joseph R.
dc.creatorOtt, Isabel M.
dc.creatorKalinich, Chaney C.
dc.creatorPetrone, Mary E.
dc.creatorCasanovas-Massana, Arnau
dc.creatorCatherine Muenker, M.
dc.creatorMoore, Adam J.
dc.creatorKlein, Jonathan
dc.creatorLu, Peiwen
dc.creatorLu-Culligan, Alice
dc.creatorJiang, Xiaodong
dc.creatorKim, Daniel J.
dc.creatorKudo, Eriko
dc.creatorMao, Tianyang
dc.creatorMoriyama, Miyu
dc.creatorOh, Ji Eun
dc.creatorPark, Annsea
dc.creatorSilva, Julio
dc.creatorSong, Eric
dc.creatorTakahashi, Takehiro
dc.creatorTaura, Manabu
dc.creatorTokuyama, Maria
dc.creatorVenkataraman, Arvind
dc.creatorWeizman, Orr-El
dc.creatorWong, Patrick
dc.creatorYang, Yexin
dc.creatorCheemarla, Nagarjuna R.
dc.creatorWhite, Elizabeth B.
dc.creatorLapidus, Sarah
dc.creatorEarnest, Rebecca
dc.creatorGeng, Bertie
dc.creatorVijayakumar, Pavithra
dc.creatorOdio, Camila
dc.creatorFournier, John
dc.creatorBermejo, Santos
dc.creatorFarhadian, Shelli
dc.creatorDela Cruz, Charles S.
dc.creatorIwasaki, Akiko
dc.creatorKo, Albert I.
dc.creatorLandry, Marie L.
dc.creatorFoxman, Ellen F.
dc.creatorGrubaugh, Nathan D.
dc.date.accessioned2020-07-16T15:00:29Z
dc.date.available2020-07-16T15:00:29Z
dc.date.created2020-07-10
dc.description.abstractenglishThe recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription–PCR (RT–qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer–probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT–qPCR analytical efficiency and sensitivity, we show that all primer–probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charité) confirmatory primer–probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer. We did not find evidence for background amplification with pre-COVID-19 samples or recent SARS-CoV-2 evolution decreasing sensitivity. Our recommendation for SARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used, to ease comparability between outcomes.spa
dc.format.extent13 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doihttps://doi.org/10.1038/s41564-020-0761-6spa
dc.identifier.issn2058-5276spa
dc.identifier.otherhttps://www.nature.com/articles/s41564-020-0761-6spa
dc.identifier.urihttps://hdl.handle.net/20.500.12010/10641
dc.publisherScience Directeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectCOVID-19spa
dc.subject.keywordSARS-CoV-2spa
dc.subject.keywordRT–qPCRspa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.titleAnalytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe setsspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.localArtículospa

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