De novo transcriptome assembly of loggerhead sea turtle nesting of the Colombian Caribbean
Fecha
2017Autor
Pinzón, Andrés
Mariño-Ramírez, Leonardo
Hernández-Fernández, Javier
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Resumen
Loggerhead sea turtle Caretta caretta is widely distributed in the oceans of tropical and subtropical latitude. This
turtle is an endangered species due to anthropic and natural factors that have decreased their population levels.
In this study, RNA sequencing and de-novo assembly of genes expressed in blood were performed. The raw
FASTQ files have been deposited on NCBI's SRA database with accession number SRX2629512. A total of 5.4 Gb
raw sequence data were obtained, corresponding to 48,257,019 raw reads. Trinity pipeline was used to perform
a de-novo assembly, we were able to identify 64,930 transcripts for female loggerhead turtle transcriptome with
an N50 of 1131 bp. The obtained transcriptome data will be useful for further studies of the physiology, biochemistry
and evolution in this species.
Palabras clave
Caretta caretta turtle; Trinity; RNAseq; Illumina; N50Enlace al recurso
http://dx.doi.org/10.1016/j.gdata.2017.06.005Colecciones
- Año 2017 [150]
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