Mostrar el registro sencillo del documento
AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI)
| dc.creator | Lancheros-Piliego, Daniel | |
| dc.creator | Hernández Fernández, Javier | |
| dc.date.accessioned | 2020-04-27T14:11:01Z | |
| dc.date.available | 2020-04-27T14:11:01Z | |
| dc.date.created | 2015 | |
| dc.identifier.other | http://www.scielo.org.co/pdf/unsc/v18n3/v18n3a06.pdf | spa |
| dc.identifier.uri | http://hdl.handle.net/20.500.12010/8978 | |
| dc.description.abstract | La tortuga cabezona Caretta caretta (Testudines: Cheloniidae) se distribuye en los océanos del mundo (en latitudes templadas, tropicales y subtropicales). Las principales playas de anidación se encuentran en la península de la Florida en Norteamérica (47.000–90.000 nidos/año en las dos últimas décadas; FWS 2013), en Brasil, Japón, en Grecia en el mar Mediterráneo, en Omán en el mar Arábigo y en la isla de Madagascar en África (Dodd 1988, European Environment Agency 2009). | spa |
| dc.format.extent | 10 páginas | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.subject | Caretta caretta | spa |
| dc.subject | High-speed PCR | spa |
| dc.subject | Cytochrome c oxidase I (COI) | spa |
| dc.subject | AMDAR | spa |
| dc.subject | Barcode | spa |
| dc.title | AMDAR y PCR-extra-rápida para la identificación de la tortuga cabezona Caretta caretta (Testudines: Cheloniidae) utilizando el gen mitocondrial citocromo c oxidasa I (COI) | spa |
| dc.title.alternative | AMDAR and PCR-Extra-fast for Molecular identification of the loggerhead sea turtle Caretta caretta (Testudines: Cheloniidae) using the mitochondrial gene cytochrome c oxidase I (COI) | eng |
| dc.subject.lemb | Tortugas -- Investigaciones | spa |
| dc.subject.lemb | ADN mitocondrial -- Investigaciones | spa |
| dc.subject.keyword | Caretta caretta | spa |
| dc.identifier.doi | 10.11144/Javeriana.SC18-3.apit | spa |
| dc.description.abstractenglish | We molecularly identified the loggerhead turtle Caretta caretta using high-speed PCR amplification and restriction analysis of mitochondrial DNA. We isolated the DNA from blood from juvenile C. caretta from Don Diego beach (Magdalena; n=4), in Islas Del Rosario (Bolívar; n=2) in the Colombian Caribbean. By using high-speed PCR amplification of mitochondrial cytochrome c oxidase I (COI), we reduced reaction by one third and obtained fragments of 650 base pairs. We analyzed the amplified IOC product using enzymes HindIII, HpyCH4III and MseI and generated an electrophoretic profile, which compared in silico to other sea turtle species sequences, revealed the loggerhead’s specific pattern. We found similarity between 97-99% with C. caretta in five of the BLAST analyzed nucleotide sequences and 92% in another. We generated a bar code for the sampled turtle information and sequences and stored them in the BOLD database. The methodology described for the identification of C. caretta is a fast and inexpensive procedure that minimizes time and improves PCR specificity. | spa |
Archivos en el documento
Este documento aparece en la(s) siguiente(s) colección(ones)
-
Año 2015 [61]