Comparative analyses of metabolomic fingerprints and cytotoxic activities of soft corals from the Colombian Caribbean
Fecha
2019Autor
Santacruz, Liliana
Thomas, Olivier P.
Duque, Carmenza
Puyana, Mónica
Tello, Edisson
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Resumen
Soft corals (Cnidaria, Anthozoa, Octocorallia) are a diverse group of marine invertebrates
that inhabit various marine environments in tropical and subtropical areas. Several species are
recognized as prolific sources of compounds with a wide array of biological activities. Recent
advances in analytical techniques, supported by robust statistical analyses, have allowed the analysis
and characterization of the metabolome present in a single living organism. In this study, a liquid
chromatography-high resolution mass spectrometry metabolomic approach was applied to analyze
the metabolite composition of 28 soft corals present in the Caribbean coast of Colombia. Multivariate
data analysis was used to correlate the chemical fingerprints of soft corals with their cytotoxic activity
against tumor cell lines for anticancer purpose. Some diterpenoids were identified as specific markers
to discriminate between cytotoxic and non-cytotoxic crude extracts of soft corals against tumor cell
lines. In the models generated from the comparative analysis of PLS-DA for tumor lines, A549 and
SiHa, the diterpene 13-keto-1,11-dolabell-3(E),7(E),12(18)-triene yielded a high score in the variable
importance in projection. These results highlight the potential of metabolomic approaches towards
the identification of cytotoxic agents against cancer of marine origin. This workflow can be useful in
several studies, mainly those that are time consuming, such as traditional bioprospecting of marine
natural products.
Palabras clave
Metabolomics; Soft-corals; Pseudoplexaura flagellosa; Diterpenes; Cytotoxic activity; LC-HRMSsEnlace al recurso
https://www.sciencedirect.com/science/article/abs/pii/S1874390018304737?via%3DihubColecciones
- Año 2019 [125]
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