Applications of phage-derived RNA-based technologies in synthetic biology
Abstract
As the most abundant biological entities with incredible diversity, bacteriophages (also known as phages) have
been recognized as an important source of molecular machines for the development of genetic-engineering tools.
At the same time, phages are crucial for establishing and improving basic theories of molecular biology. Studies
on phages provide rich sources of essential elements for synthetic circuit design as well as powerful support for
the improvement of directed evolution platforms. Therefore, phages play a vital role in the development of new
technologies and central scientific concepts. After the RNA world hypothesis was proposed and developed, novel
biological functions of RNA continue to be discovered. RNA and its related elements are widely used in many
fields such as metabolic engineering and medical diagnosis, and their versatility led to a major role of RNA in
synthetic biology. Further development of RNA-based technologies will advance synthetic biological tools as
well as provide verification of the RNA world hypothesis. Most synthetic biology efforts are based on reconstructing existing biological systems, understanding fundamental biological processes, and developing new
technologies. RNA-based technologies derived from phages will offer abundant sources for synthetic biological
components. Moreover, phages as well as RNA have high impact on biological evolution, which is pivotal for
understanding the origin of life, building artificial life-forms, and precisely reprogramming biological systems.
This review discusses phage-derived RNA-based technologies terms of phage components, the phage lifecycle,
and interactions between phages and bacteria. The significance of RNA-based technology derived from phages
for synthetic biology and for understanding the earliest stages of biological evolution will be highlighted.
Palabras clave
Bacteriophages; Synthetic biology; RNA imaging; RNA replication; Phage-bacterial interactionsLink to resource
https://doi.org/10.1016/j.synbio.2020.09.003Collections
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