Show simple item record

dc.creatorMathes, Tilo
dc.creatorKennis, John T. M.
dc.date.accessioned2020-10-09T22:55:13Z
dc.date.available2020-10-09T22:55:13Z
dc.date.created2016-01-19
dc.identifier.isbn978-2-889-19899-3
dc.identifier.issn1664-8714
dc.identifier.otherhttps://www.frontiersin.org/research-topics/3172/optogenetic-tools-in-the-molecular-spotlight
dc.identifier.urihttp://hdl.handle.net/20.500.12010/14337
dc.format.extent181 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.publisherFrontiers Media SAspa
dc.subjectScience (General)spa
dc.subjectPhotoreceptorsspa
dc.subjectFlavinsspa
dc.subjectPhytochromespa
dc.titleOptogenetic Tools in the Molecular Spotlightspa
dc.subject.lembPhotochemistry and photophysicsspa
dc.subject.lembProtein engineeringspa
dc.subject.lembComputational modelingspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.localAbierto (Texto Completo)spa
dc.subject.keywordOptogeneticsspa
dc.subject.keywordOpsinsspa
dc.subject.keywordSpectroscopyspa
dc.identifier.doi10.3389/978-2-88919-899-3
dc.relation.referencesMathes, T., Kennis, J. T. M., eds. (2016). Optogenetic Tools in the Molecular Spotlight. Lausanne: Frontiers Media. doi: 10.3389/978-2-88919-899-3
dc.description.abstractenglishThe rise of optogenetics as a standard technique to non-invasively probe and monitor biological function created an immense interest in the molecular function of photosensory proteins. These photoreceptors are usually protein/pigment complexes that translate light into biological information and have become essential tools in cell biology and neurobiology as their function is genetically encoded and can be conveniently delivered into a given cell. Like for fluorescent proteins that quickly became invaluable as genetically encodable reporters in microscopy and imaging, variants of photosensory proteins with customized sensitivity and functionality are nowadays in high demand. In this ebook we feature reviews and original research on molecular approaches from synthetic biology and molecular spectroscopy to computational molecular modelling that all aspire to elucidate the molecular prerequisites for the photosensory function of the given proteins. The principle property of changing activity of biological function simply by application of light is not only very attractive for cell biology, it also offers unique opportunities for molecular studies as excitation can be controlled with high time precision. Especially in spectroscopy the usually fully reversible photoactivation of photosensory proteins allows researchers to to perform time resolved studies with up to femtosecond resolution. In addition, functional variants can be investigated and quickly screened in common biochemical experiments. The insights that are obtained by the here presented various yet complementary methods will ultimately allow us write the script for a molecular movie from excitation of the protein by a photon to activation of its biological function. Such deep understanding does not only provide unique insights into the dynamics of protein function, it will also ultimately enable us to rationally design novel optogenetic tools to be used in cell biology and therapy.spa
dc.type.coarhttp://purl.org/coar/resource_type/c_2f33spa


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record