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dc.creatorChi, Xiaojing
dc.creatorLiu, Xiuying
dc.creatorWang, Conghui
dc.creatorZhang, Xinhui
dc.creatorLi, Xiang
dc.creatorHou, Jianhua
dc.creatorRen, Lili
dc.creatorJin, Qi
dc.creatorWang, Jianwei
dc.creatorYang, Wei
dc.date.accessioned2020-09-17T21:57:49Z
dc.date.available2020-09-17T21:57:49Z
dc.date.created2020
dc.identifier.issn2041-1723spa
dc.identifier.otherhttps://doi.org/10.1038/s41467-020-18387-8spa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/13397
dc.description.abstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spreads worldwide and leads to an unprecedented medical burden and lives lost. Neutralizing antibodies provide efficient blockade for viral infection and are a promising category of biological therapies. Here, using SARS-CoV-2 spike receptor-binding domain (RBD) as a bait, we generate a panel of humanized single domain antibodies (sdAbs) from a synthetic library. These sdAbs reveal binding kinetics with the equilibrium dissociation constant (KD) of 0.99–35.5 nM. The monomeric sdAbs show half maximal neutralization concentration (EC50) of 0.0009–0.07 µg/mL and 0.13–0.51 µg/mL against SARS-CoV-2 pseudotypes, and authentic SARS-CoV-2, respectively. Competitive ligand-binding experiments suggest that the sdAbs either completely block or significantly inhibit the association between SARS-CoV-2 RBD and viral entry receptor ACE2. Fusion of the human IgG1 Fc to sdAbs improve their neutralization activity by up to ten times. These results support neutralizing sdAbs as a potential alternative for antiviral therapies.spa
dc.format.extent7 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherNature cammunicationsspa
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectSARS-CoV-2spa
dc.subjectDomain antibodiesspa
dc.titleHumanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domainspa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.rights.localAbierto (Texto Completo)spa
dc.identifier.doihttps://doi.org/10.1038/s41467-020-18387-8spa
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1spa


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