Quantitative proteomic analysis of the tizoxanide efect in vero cells
Date
2020Author
Yamamoto, K.A.
Blackburn, K.
Migowsk, E.
Goshe, M. B.
Brown, D. T.
Ferreira, D. F.
Soares, M. R.
Metadata
Show full item record
Documentos PDF
Abstract
Nitazoxanide (NTZ) is efective against helminths and numerous microorganisms, including bacteria
and viruses. In vivo, NTZ is metabolized into Tizoxanide (TIZ), which is the active circulating
metabolite. With the emergence of SARS-Cov-2 as a Pandemic agent, NTZ became one of the
molecules already approved for human use to engage clinical trials, due to results in vitro showing
that NTZ was highly efective against the SARS-Cov-2, agent of COVID-19.There are currently several
ongoing clinical trials mainly in the USA and Brazil involving NTZ due not only to the in vitro results,
but also for its long-known safety. Here, we study the response ofVero cells toTIZ treatment and
unveil possible mechanisms for its antimicrobial efect, using a label-free proteomic approach (LC/
MS/MS) analysis to compare the proteomic profle between untreated- andTIZ-treated cells. Fifteen
diferentially expressed proteins were observed related to various biological processes, including
translation, intracellular trafcking, RNA processing and modifcation, and signal transduction.The
broad antimicrobial range ofTIZ points towards its overall efect in lowering cell metabolism and
RNA processing and modifcation.The decreased levels of FASN, HNRNPH and HNRNPK with the
treatment appear to be important for antiviral activity.
Palabras clave
Proteomic analysis; Tizoxanide; Vero cellsLink to resource
https://doi.org/10.1038/s41598-020-71634-2Collections
Estadísticas Google Analytics
Comments
Respuesta Comentario Repositorio Expeditio
Gracias por tomarse el tiempo para darnos su opinión.