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dc.creatorMurphy, Emma J.
dc.creatorMasterson, Claire
dc.creatorRezoagli, Emanuele
dc.creatorO'Toole, Daniel
dc.creatorMajor, Ian
dc.creatorStack, Gary D.
dc.creatorLynch, Mark
dc.creatorLaffey, John G.
dc.creatorRowan, Neil J.
dc.date.accessioned2020-08-03T20:45:22Z
dc.date.available2020-08-03T20:45:22Z
dc.date.created2020
dc.identifier.issn0048-9697spa
dc.identifier.otherhttps://doi.org/10.1016/j.scitotenv.2020.139330spa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/11568
dc.description.abstractCoronavirus pneumonia is accompanied by rapid virus replication, where a large number of inflammatory cell infiltration and cytokine storm may lead to acute lung injury, acute respiratory distress syndrome (ARDS) and death. The uncontrolled release of pro-inflammatory cytokines, including interleukin (IL)-1β and IL-6, is associated with ARDS. This constituted the first study to report on the variability in physicochemical properties of βglucans extracts from the same edible mushroom Lentinus edodes on the reduction of these pro-inflammatory cytokines and oxidative stress. Specifically, the impact on the immunomodulatory and cytoprotective properties of our novel in ‘house’ (IH-Lentinan, IHL) and a commercial (Carbosynth-Lentinan, CL) Lentinan extract were investigated using in vitro models of lung injury and macrophage phagocytosis. CL comprised higher amounts of αglucans and correspondingly less β-glucans. The two lentinan extracts demonstrated varying immunomodulatory activities. Both Lentinan extracts reduced cytokine-induced NF-κB activation in human alveolar epithelial A549 cells, with the IHL extract proving more effective at lower doses. In contrast, in activated THP-1 derivedspa
dc.format.extent10 páginasspa
dc.format.mimetypeimage/jepgspa
dc.publisherScience of the Total Environmenteng
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectβ-Glucansspa
dc.subjectMedicinal mushroomspa
dc.subjectCOVID-19spa
dc.subjectImmunomodulationspa
dc.titleβ-Glucan extracts from the same edible shiitake mushroom Lentinus edodes produce differential in-vitro immunomodulatory and pulmonary cytoprotective effects — Implications for coronavirus disease (COVID-19) immunotherapiesspa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.identifier.doihttps://doi.org/10.1016/j.scitotenv.2020.139330spa
dc.description.abstractenglishmacrophages, the CL extract more effectively attenuated pro-inflammatory cytokine production (TNF-α, IL-8, IL2, IL-6, IL-22) as well as TGF-β and IL-10. The CL extract attenuated oxidative stress-induced early apoptosis, while the IHL extract attenuated late apoptosis. Our findings demonstrate significant physicochemical differences between Lentinan extracts, which produce differential in vitro immunomodulatory and pulmonary cytoprotective effects that may also have positive relevance to candidate COVID-19 therapeutics targeting cytokine storm.spa


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