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dc.creatorWing-Ho Chu, Allen
dc.creatorChan, Wan-Mui
dc.creatorIp, Jonathan Daniel
dc.creatorChik-Yan Yip, Cyril
dc.creatorFuk-Woo Chan, Jasper
dc.creatorYuen, Kwok-Yung
dc.creatorKai-Wang To, Kelvin
dc.date.accessioned2020-07-28T20:27:25Z
dc.date.available2020-07-28T20:27:25Z
dc.date.created2020
dc.identifier.issn1386-6532spa
dc.identifier.otherhttps://doi.org/10.1016/j.jcv.2020.104519spa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/11307
dc.description.abstractBackground: The severe shortage of nucleic acid extraction kits during the current COVID-19 pandemic represents a key limiting factor in testing capacity. Objectives: This study compared the results of SARS-CoV-2 RT-PCR using different simple nucleic acid extraction methods on nasopharyngeal and saliva specimens. Study design: Fifty nasopharyngeal swab and saliva specimens previously tested positive for SARS-CoV-2 were retrieved. Three different methods of nucleic acid extraction were compared. The first method involves incubating the specimen with proteinase K, and then heat treatment at 98 °C for 5 min (PKH); the second method involves heat treatment at 98 °C for 5 min without proteinase K pre-incubation (heat only); the third method involves no pre-processing steps (direct). The products from all 3 methods were tested by SARS-CoV-2 RT-PCR. Results: PKH had significantly higher positive rate in SARS-CoV-2 RT-PCR (80 %) than those of heat only (58 %; P = 0.001) or direct (56 %; P = 0.002). The median Ct value was significantly earlier for PKH (median Ct: 37.0, IQR 31.7–40) than that of heat only (median Ct: 40, IQR 36.2–41; P < 0.0001) and direct (median Ct, 37.5; IQR 33.9–41.0; P = 0.0049). Subgroup analysis showed that PKH had higher detection rate, lower limit of detection and earlier Ct values than the other two groups for both NPS and saliva specimens. Conclusions: PKH pre-processing resulted in the highest detection rate of SARS-CoV-2 by RT-PCR, and represents an alternative method for nucleic acid extraction when commercial extraction kits are not available.spa
dc.format.extent4 páginasspa
dc.format.mimetypeimage/jepgspa
dc.publisherJournal of Clinical Virologyeng
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectSARS-CoV-2spa
dc.subjectProteinase Kspa
dc.subjectDetectionspa
dc.subjectRT-PCRspa
dc.subjectCOVID-19spa
dc.subjectNucleic acid extractionspa
dc.titleEvaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kitsspa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.identifier.doihttps://doi.org/10.1016/j.jcv.2020.104519spa


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