Immunogenicity of a DNA vaccine candidate for COVID-19
Date
2020-05-20Author
Smith, Trevor R.F.
Patel, Ami
Ramos, Stephanie
Elwood, Dustin
Zhu, Xizhou
Yan, Jian
Gary, Ebony N.
Walker, Susanne N.
Schultheis, Katherine
Purwar, Mansi
Xu, Ziyang
Walters, Jewell
Bhojnagarwala, Pratik
Yang, Maria
Chokkalingam, Neethu
Pezzoli, Patrick
Parzych, Elizabeth
Reuschel, Emma L.
Doan, Arthur
Tursi, Nicholas
Vasquez, Miguel
Choi, Jihae
Tello-Ruiz, Edgar
Maricic, Igor
Bah, Mamadou A.
Wu, Yuanhan
Amante, Dinah
Park, Daniel H.
Dia, Yaya
Ali, Ali Raza
Zaidi, Faraz I.
Generotti, Alison
Kim, Kevin Y.
Herring, Timothy A.
Reeder, Sophia
Andrade, Viviane M.
Buttigieg, Karen
Zhao, Gan
Wu, Jiun Ming
Li, Dan
Bao, Linlin
Liu, Jiangning
Deng, Wei
Qin, Chuan
Brown, Ami Shah
Khoshnejad, Makan
Wang, Nianshuang
Chu, Jacqueline
Wrapp, Daniel
McLellan, Jason S.
Muthumani, Kar
Wang, Bin
Carroll, Miles W.
Kim, J. Joseph
Boyer, Jean
Kulp, Daniel W.
Humeau, Laurent M.P.F.
Weiner, David B.
Broderick, Kate E.
Metadata
Show full item record
Documentos PDF
Summary in foreign language
The coronavirus family member, SARS-CoV-2 has been identified as the causal agent for the pandemic viral pneumonia disease, COVID-19. At this time, no vaccine is available to control further dissemination of the disease. We have previously engineered a synthetic DNA vaccine targeting the MERS coronavirus Spike (S) protein, the major surface antigen of coronaviruses, which is currently in clinical study. Here we build on this prior experience to generate a synthetic DNA-based vaccine candidate targeting SARS-CoV-2 S protein. The engineered construct, INO-4800, results in robust expression of the S protein in vitro. Following immunization of mice and guinea pigs with INO-4800 we measure antigen-specific T cell responses, functional antibodies which neutralize the SARS-CoV-2 infection and block Spike protein binding to the ACE2 receptor, and biodistribution of SARS-CoV-2 targeting antibodies to the lungs. This preliminary dataset identifies INO-4800 as a potential COVID-19 vaccine candidate, supporting further translational study.
Palabras clave
VacunaciónLink to resource
https://www.nature.com/articles/s41467-020-16505-0Collections
Estadísticas Google Analytics
Comments
Respuesta Comentario Repositorio Expeditio
Gracias por tomarse el tiempo para darnos su opinión.