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Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice
dc.creator | McKay, Paul F | |
dc.creator | Hu, Kai | |
dc.creator | Blakney, Anna K | |
dc.creator | Samnuan, Karnyart | |
dc.creator | Brown, Jonathan C | |
dc.creator | Penn, Rebecca | |
dc.creator | Zhou, Jie | |
dc.creator | Bouton, Clément R | |
dc.creator | Rogers, Paul | |
dc.creator | Polra, Krunal | |
dc.creator | Lin, Paulo J C | |
dc.creator | Barbosa, Christopher | |
dc.creator | Tam, Ying K | |
dc.creator | Barclay, Wendy S | |
dc.creator | Shattock, Robin J | |
dc.date.accessioned | 2020-07-22T20:27:27Z | |
dc.date.available | 2020-07-22T20:27:27Z | |
dc.date.created | 2020-07-09 | |
dc.identifier.issn | 2041-1723 | spa |
dc.identifier.other | https://www.nature.com/articles/s41467-020-17409-9#article-info | spa |
dc.identifier.uri | http://hdl.handle.net/20.500.12010/10978 | |
dc.format.extent | 7 páginas | spa |
dc.format.mimetype | application/pdf | spa |
dc.publisher | Science Direct | eng |
dc.source | reponame:Expeditio Repositorio Institucional UJTL | spa |
dc.source | instname:Universidad de Bogotá Jorge Tadeo Lozano | spa |
dc.subject | Vacuna de nanopartículas lipídicas | spa |
dc.title | Self-amplifying RNA SARS-CoV-2 lipid nanoparticle vaccine candidate induces high neutralizing antibody titers in mice | spa |
dc.type.local | Artículo | spa |
dc.subject.lemb | Síndrome respiratorio agudo grave | spa |
dc.subject.lemb | COVID-19 | spa |
dc.subject.lemb | SARS-CoV-2 | spa |
dc.subject.lemb | Coronavirus | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.type.hasversion | info:eu-repo/semantics/acceptedVersion | spa |
dc.subject.keyword | RNA SARS-CoV-2 | spa |
dc.identifier.doi | https://doi.org/10.1038/s41467-020-17409-9 | spa |
dc.description.abstractenglish | The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle (LNP) as a vaccine. We observe remarkably high and dose-dependent SARS-CoV-2 specific antibody titers in mouse sera, as well as robust neutralization of both a pseudo-virus and wild-type virus. Upon further characterization we find that the neutralization is proportional to the quantity of specific IgG and of higher magnitude than recovered COVID-19 patients. saRNA LNP immunizations induce a Th1-biased response in mice, and there is no antibody-dependent enhancement (ADE) observed. Finally, we observe high cellular responses, as characterized by IFN-γ production, upon re-stimulation with SARS-CoV-2 peptides. These data provide insight into the vaccine design and evaluation of immunogenicity to enable rapid translation to the clinic. | spa |