Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics
Date
2020Author
Lu, Shuhan
Li, Fang
Chen, Qiubing
Wu, Jing
Duan, Junyi
Lei, Xinlin
Zhang, Ying
Zhao, Dongming
Bu, Zhigao
Yin, Hao
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Abstract
African swine fever virus (ASFV) is a dsDNA virus responsible for a severe, highly contagious, and lethal disease
affecting both domestic and wild pigs. ASFV has brought enormous economic loss to a number of countries, and
effective vaccine and therapy are still lacking. Therefore, a rapid, sensitive, and field-deployable detection of ASFV is
important for disease surveillance and control. Herein, we developed a Cas12a-mediated portable paper assay to
rapidly and precisely detect ASFV. We identified a robust set of crRNAs that recognized the highly conserved region of
essential ASFV genes. The Cas12a-mediated detection assay showed low tolerance for mismatch mutations, and no
cross-reactivity against other common swine pathogens. We further developed a paper-based assay to allow
instrument-free detection of ASFV. Specifically, we applied gold nanoparticle–antibody conjugate to engineer
homemade strips and combined it with Cas12a-mediated ASFV detection. This portable paper, instrument-free
diagnostics, faithfully detected ASFV in swine samples, showing comparable sensitivity to the traditionally instrumentdependent qPCR method. Taking together, we developed a highly sensitive, instant, and economic Cas12a-mediated
paper diagnostics of ASFV, with a great application potential for monitoring ASFV in the field.
Link to resource
https://doi.org/10.1038/s41421-020-0151-5Collections
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