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dc.creatorSchaefer, Inga-Marie
dc.creatorPadera, Robert F.
dc.creatorSolomon, Isaac H.
dc.creatorKanjilal, Sanjat
dc.creatorHammer, Mark M.
dc.creatorHornick, Jason L.
dc.creatorSholl, Lynette M.
dc.date.accessioned2020-07-16T18:35:51Z
dc.date.available2020-07-16T18:35:51Z
dc.date.created2020-06-19
dc.identifier.issn1530-0285 (online)spa
dc.identifier.otherhttps://www.nature.com/articles/s41379-020-0595-zspa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/10684
dc.format.extent11 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.publisherModern Pathologyeng
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectairwaysspa
dc.subjectacute respiratory distressspa
dc.titleIn situ detection of SARS-CoV-2 in lungs and airways of patients with COVID-19spa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.identifier.doihttps://doi.org/10.1038/s41379-020-0595-zspa
dc.description.abstractenglishCoronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 has led to a global public health crisis. In elderly individuals and those with comorbidities, COVID-19 is associated with high mortality, frequently caused by acute respiratory distress syndrome. We examine in situ expression of SARS-CoV-2 in airways and lung obtained at autopsy of individuals with confirmed COVID-19 infection. Seven autopsy cases (male, N = 5; female, N = 2) with reverse transcriptase-polymerase chain reaction (RT-PCR)-confirmed SARS-CoV-2 infection and a median age of 66 years (range, 50–77 years) were evaluated using a rabbit polyclonal antibody against SARS Nucleocapsid protein in correlation with clinical parameters. The median time from symptom onset to death was 9 days (range, 6–31 days), from hospitalization 7 days (range, 1–21 days), from positive RT-PCR 7 days (range, 0–18 days), and from intensive care unit admission defining onset of respiratory failure 3 days (range, 1–18 days). Chest imaging identified diffuse airspace disease in all patients corresponding to acute and (N = 5) or organizing (N = 2) diffuse alveolar damage (DAD) on histologic examination. Among five patients with acute-phase DAD (≤7 days from onset of respiratory failure), SARS-CoV-2 was detected in pulmonary pneumocytes and ciliated airway cells (N = 5), and in upper airway epithelium (N = 2). In two patients with organizing DAD (>14 days from onset of respiratory failure), no virus was detected in lungs or airways. No endothelial cell infection was observed. The findings suggest that SARS-CoV-2 infection of epithelial cells in lungs and airways of patients with COVID-19 who developed respiratory failure can be detected during the acute phase of lung injury and is absent in the organizing phase.spa


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