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dc.creatorAckerman, Cheri M.
dc.creatorMyhrvold, Cameron
dc.creatorThakku, Sri Gowtham
dc.creatorFreije, Catherine A.
dc.creatorMetsky, Hayden C.
dc.creatorYang, David K.
dc.creatorYe, Simon H.
dc.creatorBoehm, Chloe K.
dc.creatorKosoko-Thoroddsen, Tinna Sólveig F.
dc.creatorKehe, Jared
dc.creatorNguyen, Tien G.
dc.creatorCarter, Amber
dc.creatorKulesa, Anthony
dc.creatorBarnes, John R.
dc.creatorDugan, Vivien G.
dc.creatorHung, Deborah T.
dc.creatorBlainey, Paul C.
dc.creatorSabeti, Pardis C.
dc.date.accessioned2020-07-16T15:31:43Z
dc.date.available2020-07-16T15:31:43Z
dc.date.created2020-04-29
dc.identifier.issn1476-4687spa
dc.identifier.otherhttps://www.nature.com/articles/s41586-020-2279-8spa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/10652
dc.format.extent27 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.publisherScience Directeng
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectDetección de ácido nucleicospa
dc.titleMassively multiplexed nucleic acid detection with Cas13spa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.subject.keywordNucleic acid detectionspa
dc.identifier.doihttps://doi.org/10.1038/s41586-020-2279-8spa
dc.description.abstractenglishThe great majority of globally circulating pathogens go undetected, undermining patient care and hindering outbreak preparedness and response. To enable routine surveillance and comprehensive diagnostic applications, there is a need for detection technologies that can scale to test many samples1,2,3 while simultaneously testing for many pathogens4,5,6. Here, we develop Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (CARMEN), a platform for scalable, multiplexed pathogen detection. In the CARMEN platform, nanolitre droplets containing CRISPR-based nucleic acid detection reagents7 self-organize in a microwell array8 to pair with droplets of amplified samples, testing each sample against each CRISPR RNA (crRNA) in replicate. The combination of CARMEN and Cas13 detection (CARMEN–Cas13) enables robust testing of more than 4,500 crRNA–target pairs on a single array. Using CARMEN–Cas13, we developed a multiplexed assay that simultaneously differentiates all 169 human-associated viruses with at least 10 published genome sequences and rapidly incorporated an additional crRNA to detect the causative agent of the 2020 COVID-19 pandemic. CARMEN–Cas13 further enables comprehensive subtyping of influenza A strains and multiplexed identification of dozens of HIV drug-resistance mutations. The intrinsic multiplexing and throughput capabilities of CARMEN make it practical to scale, as miniaturization decreases reagent cost per test by more than 300-fold. Scalable, highly multiplexed CRISPR-based nucleic acid detection shifts diagnostic and surveillance efforts from targeted testing of high-priority samples to comprehensive testing of large sample sets, greatly benefiting patients and public health.spa


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