Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
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Date
2020-07-10Author
Vogels, Chantal B. F.
Brito, Anderson F.
Wyllie, Anne L.
Fauver, Joseph R.
Ott, Isabel M.
Kalinich, Chaney C.
Petrone, Mary E.
Casanovas-Massana, Arnau
Catherine Muenker, M.
Moore, Adam J.
Klein, Jonathan
Lu, Peiwen
Lu-Culligan, Alice
Jiang, Xiaodong
Kim, Daniel J.
Kudo, Eriko
Mao, Tianyang
Moriyama, Miyu
Oh, Ji Eun
Park, Annsea
Silva, Julio
Song, Eric
Takahashi, Takehiro
Taura, Manabu
Tokuyama, Maria
Venkataraman, Arvind
Weizman, Orr-El
Wong, Patrick
Yang, Yexin
Cheemarla, Nagarjuna R.
White, Elizabeth B.
Lapidus, Sarah
Earnest, Rebecca
Geng, Bertie
Vijayakumar, Pavithra
Odio, Camila
Fournier, John
Bermejo, Santos
Farhadian, Shelli
Dela Cruz, Charles S.
Iwasaki, Akiko
Ko, Albert I.
Landry, Marie L.
Foxman, Ellen F.
Grubaugh, Nathan D.
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Summary in foreign language
The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical and public health interventions. Currently, several quantitative reverse transcription–PCR (RT–qPCR) assays are being used by clinical, research and public health laboratories. However, it is currently unclear whether results from different tests are comparable. Our goal was to make independent evaluations of primer–probe sets used in four common SARS-CoV-2 diagnostic assays. From our comparisons of RT–qPCR analytical efficiency and sensitivity, we show that all primer–probe sets can be used to detect SARS-CoV-2 at 500 viral RNA copies per reaction. The exception for this is the RdRp-SARSr (Charité) confirmatory primer–probe set which has low sensitivity, probably due to a mismatch to circulating SARS-CoV-2 in the reverse primer. We did not find evidence for background amplification with pre-COVID-19 samples or recent SARS-CoV-2 evolution decreasing sensitivity. Our recommendation for SARS-CoV-2 diagnostic testing is to select an assay with high sensitivity and that is regionally used, to ease comparability between outcomes.
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COVID-19Link to resource
https://www.nature.com/articles/s41564-020-0761-6Collections
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