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dc.creatorMa, Chunlong
dc.creatorSacco, Michael Dominic
dc.creatorHurst, Brett
dc.creatorTownsend, Julia Alma
dc.creatorHu, Yanmei
dc.creatorSzeto, Tommy
dc.creatorZhang, Xiujun
dc.creatorTarbet, Bart Bart
dc.creatorMarty, Michael Thomas
dc.creatorChen, Yu
dc.creatorWang, Jun
dc.date.accessioned2020-07-15T15:15:25Z
dc.date.available2020-07-15T15:15:25Z
dc.date.created2020-06-15
dc.identifier.issn1748-7838 (online)spa
dc.identifier.otherhttps://www.nature.com/articles/s41422-020-0356-z#auth-2spa
dc.identifier.urihttp://hdl.handle.net/20.500.12010/10549
dc.format.extent15 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.publisherCell Researcheng
dc.sourcereponame:Expeditio Repositorio Institucional UJTLspa
dc.sourceinstname:Universidad de Bogotá Jorge Tadeo Lozanospa
dc.subjectBoceprevirspa
dc.subjectcalpain inhibitors IIspa
dc.subjectGC-376spa
dc.titleBoceprevir, GC-376, and calpain inhibitors II, XII inhibit SARS-CoV-2 viral replication by targeting the viral main proteasespa
dc.type.localArtículospa
dc.subject.lembSíndrome respiratorio agudo gravespa
dc.subject.lembCOVID-19spa
dc.subject.lembSARS-CoV-2spa
dc.subject.lembCoronavirusspa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersionspa
dc.identifier.doihttps://doi.org/10.1038/s41422-020-0356-zspa
dc.description.abstractenglishA new coronavirus SARS-CoV-2, also called novel coronavirus 2019 (2019-nCoV), started to circulate among humans around December 2019, and it is now widespread as a global pandemic. The disease caused by SARS-CoV-2 virus is called COVID-19, which is highly contagious and has an overall mortality rate of 6.35% as of May 26, 2020. There is no vaccine or antiviral available for SARS-CoV-2. In this study, we report our discovery of inhibitors targeting the SARS-CoV-2 main protease (Mpro). Using the FRET-based enzymatic assay, several inhibitors including boceprevir, GC-376, and calpain inhibitors II, and XII were identified to have potent activity with single-digit to submicromolar IC50 values in the enzymatic assay. The mechanism of action of the hits was further characterized using enzyme kinetic studies, thermal shift binding assays, and native mass spectrometry. Significantly, four compounds (boceprevir, GC-376, calpain inhibitors II and XII) inhibit SARS-CoV-2 viral replication in cell culture with EC50 values ranging from 0.49 to 3.37 µM. Notably, boceprevir, calpain inhibitors II and XII represent novel chemotypes that are distinct from known substrate-based peptidomimetic Mpro inhibitors. A complex crystal structure of SARS-CoV-2 Mpro with GC-376, determined at 2.15 Å resolution with three protomers per asymmetric unit, revealed two unique binding configurations, shedding light on the molecular interactions and protein conformational flexibility underlying substrate and inhibitor binding by Mpro. Overall, the compounds identified herein provide promising starting points for the further development of SARS-CoV-2 therapeutics.spa


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