Publicación: Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax
| dc.contributor.author | Hernández-Fernández, Javier Adolfo | |
| dc.creator | Gómez, Sylvia | |
| dc.creator | Mateus, Ana Constanza | |
| dc.creator | Zimmermann, Barbara H. | |
| dc.date.accessioned | 2024-04-03T10:02:20Z | |
| dc.date.available | 2024-04-03T10:02:20Z | |
| dc.date.created | 2000-11 | |
| dc.description.abstract | El gorgojo andino de la papa, Premnotrypes vorax, un insecto del orden Coleoptera, es una de las principales causas de daño a los cultivos de papa en las regiones andinas de América del Sur. Las proteínas Cry insecticidas de Bacillus thuringiensis son pesticidas biológicos útiles, y algunas son tóxicas para los insectos coleópteros. Se sobreexpresó la proteína Cry3Aa recombinante etiquetada con histidina en células hospedadoras de Escherichia coli. La proteína recombinante se solubilizó a un pH alto con urea, se purificó utilizando resina de afinidad de nitrilotriacetato de Ni^21^ y se dializó para bajar el pH y eliminar la urea. Se realizaron bioensayos con un medio para insectos cuya superficie estaba impregnada con 70 mg/mL de toxinas nativas o recombinantes purificadas. Las larvas de primer estadio expuestas al medio tratado con toxina durante 5 días exhibieron mortalidades del 57% (Cry3Aa nativa) al 52% (Cry3Aa recombinante). Las proteínas Cry3Aa nativas y recombinantes purificadas parecieron ser igualmente tóxicas para el gorgojo andino de la papa. | spa |
| dc.description.abstractenglish | The Andean potato weevil, Premnotrypes vorax, an insect of the order Coleoptera, is a major cause of damage to potato crops in the Andean regions of South America. The insecticidal Cry proteins from Bacillus thuringiensis are useful biological pesticides, and some are toxic to Coleopteran insects. We overexpressed recombinant, histidine-tagged Cry3Aa protein in Escherichia coli host cells. The recombinant protein was solubilized at high pH with urea, purified using Ni21-nitrilo-triacetic acid affinity resin, and dialysed to lower pH and remove urea. Bioassays were performed with an insect media whose surface was spread with 70 mg/mL purified native or recombinant toxins. First instar larvae exposed to toxin treated media for 5 days exhibited mortalities from 57% (native Cry3Aa) to 52% (recombinant Cry3Aa). Purified native and recombinant Cry3Aa proteins appeared to be equally toxic to the Andean potato weevil. | spa |
| dc.description.hashtag | #Cry3Aa | spa |
| dc.description.hashtag | #Recombinante | spa |
| dc.description.hashtag | #Insecticida | spa |
| dc.description.hashtag | #Gorgojoandino | spa |
| dc.description.hashtag | #Papa | spa |
| dc.description.hashtag | #Premnotrypesvorax | spa |
| dc.format.extent | 653–656 páginas | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.other | https://www.researchgate.net/publication/12210874_Recombinant_Cry3Aa_Has_Insecticidal_Activity_against_the_Andean_Potato_Weevil_Premnotrypes_vorax | spa |
| dc.identifier.uri | https://hdl.handle.net/20.500.12010/34173 | |
| dc.language.iso | spa | spa |
| dc.publisher | Biochemical and Biophysical Research Communications Volumen 279 | spa |
| dc.relation.references | 1. Alcazar, J., and Cisneros, F. (posted 1996) Integrated Management for Andean potato weevils in pilot units. The International Potato Center (CIP). Available at http://www.cipotato.org/new/ WebProRep96/program4/prog43.htm. | spa |
| dc.relation.references | 2. Bravo, A. (1997) Phylogenetic relationships of Bacillus thuringiensis delta-endotoxin family proteins and their functional domains. J. Bacteriol. 179, 2793–2801 | spa |
| dc.relation.references | 3. Hofte, H., and Whiteley, H. R. (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53, 242–255. | spa |
| dc.relation.references | 4. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., and Dean, D. H. (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62, 775–806. | spa |
| dc.relation.references | 5. Crickmore, N. 21 (posted July 1999) Bt Toxin Nomenclature. Available at http://www.biols.susx.ac.uk/Home/Neil_Crickmore/ Bt/index.hml. | spa |
| dc.relation.references | 7. Hofte, H., Seurinck, J., Van Houtven, A., and Vaeck, M. (1987) Nucleotide sequence of a gene encoding an insecticidal protein of Bacillus thuringiensis var. tenebrionis toxic against Coleoptera. Nucleic. Acids. Res. 15, 7183. | spa |
| dc.relation.references | 8. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. | spa |
| dc.relation.references | 9. Koller, C. N., Bauer, L. S., and Hollingworth, R. M. (1992) Characterization of the pH-mediated solubility of Bacillus thuringiensis var. san diego native delta endotoxin crystal. Biochim. Biophys. Res. Comm. 184, 692–699. | spa |
| dc.relation.references | 10. Grayson, D. R., Lee, L., and Evans, D. R. (1985) Immunochemical analysis of the domain structure of CAD, the multifunctional protein that initiates pyrimidine biosynthesis in mammalian cells. J. Biol. Chem. 260, 15840–15849. | spa |
| dc.relation.references | 11. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685. | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.local | Abierto (Texto Completo) | spa |
| dc.subject | Cry3Aa | spa |
| dc.subject | Cry3A | spa |
| dc.subject | Premnotrypes vorax | spa |
| dc.subject | Gorgojo andino de la papa | spa |
| dc.subject | Bacillus thuringiensis | spa |
| dc.subject.keyword | Cry3Aa | spa |
| dc.subject.keyword | Cry3A | spa |
| dc.subject.keyword | Premnotrypes vorax | spa |
| dc.subject.keyword | Andean potato weevil | spa |
| dc.subject.keyword | Bacillus thuringiensis | spa |
| dc.subject.lemb | Gorgojo andino de la papa -- Premnotrypes vorax -- Control biológico | spa |
| dc.subject.lemb | Proteínas Cry -- Pesticidas biológicos | spa |
| dc.subject.lemb | Manejo integrado de plagas -- Métodos de contol | spa |
| dc.subject.lemb | Cultivo de papa -- Biotecnología agrícola | spa |
| dc.title | Recombinant Cry3Aa has insecticidal activity against the andean potato weevil, Premnotrypes Vorax | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 22c0e1d7-3e57-4ff1-8db2-7f60bee18bfc | |
| relation.isAuthorOfPublication.latestForDiscovery | 22c0e1d7-3e57-4ff1-8db2-7f60bee18bfc |
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